Open AccessCulture of human ovarian tissue in xeno-free conditions using laminin components of the human ovarian extracellular matrix
Author(s) -
Jie Hao,
Astrud R. Tuck,
Chandan Prakash,
Anastasios Damdimopoulos,
Marcus Sjödin,
Johan Lindberg,
Boel Niklasson,
Karin Pettersson,
Outi Hovatta,
Pauliina Damdimopoulou
Publication year - 2020
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-020-01886-4
Subject(s) - matrigel , laminin , extracellular matrix , ovarian cortex , ovarian follicle , biology , ovary , andrology , subculture (biology) , microbiology and biotechnology , cell culture , endocrinology , ovarian tissue , medicine , genetics , botany
Our purpose was to identify human ovarian extracellular matrix (ECM) components that would support in vitro culture of human ovarian tissue and be compatible with possible future clinical applications. We characterized ovarian expression of laminins and selected three laminin tripeptides for culture experiments to be compared with Matrigel, an undefined and animal-based mixture of ECM components.