English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

With the increase in antimicrobial resistance, fast reporting of antimicrobial susceptibility testing (AST) results is becoming increasingly important. EUCAST developed a method for rapid AST (RAST) directly from the broth of positive blood cultures (BC). Inhibition zones are read after 4, 6, and 8 h, with specific breakpoints per time point. We evaluated the RAST method based on EUCAST disk diffusion methodology with inoculation of BC broth using WASPLab ®  (inclusive Colibrí ™  and Radian ® ). Forty-nine non-duplicate strains were tested:  Escherichia coli n  = 17,  Klebsiella pneumoniae n  = 7,  Pseudomonas aeruginosa n  = 4,  Acinetobacter baumannii n  = 2,  Staphylococcus aureus n  = 10,  Enterococcus faecalis n  = 6, and  Enterococcus faecium n  = 3. Results were compared to direct AST and standardized AST. Good categorical agreement was obtained at all time points for all groups, except  P. aeruginosa . RAST cut-offs for extended-spectrum β-lactamase (ESBL)-producing  Enterobacterales  enabled the detection of all included ESBL isolates ( n  = 5) at all time points, except for 1  E. coli  ESBL after 4 h. RAST cut-offs for carbapenemase-producing  Enterobacterales  enabled the detection of only one carbapenemase after 6 h. However, all carbapenemases ( n  = 3) were correctly detected after 8 h. Two methicillin-resistant  S. aureus  were included; both were correctly categorized as cefoxitin-resistant at 6 and 8 h. At 4 h, there was insufficient growth for inhibition zone interpretation. EUCAST RAST is a fast supplementary tool for direct AST of positive BC. WASPLab ®  provides a significant advantage as pictures are made automatically implicating that we are not strictly bound to the time points for inhibition zone interpretation.

Validation of Rapid Antimicrobial Susceptibility Testing directly from blood cultures using WASPLab®, including Colibrí™ and Radian® in-Line Carousel