Open AccessBone marrow‐derived multipotent mesenchymal stromal cells from horses after euthanasia
Author(s) -
Schröck Carmen,
Eydt Carina,
Geburek Florian,
Kaiser Lena,
Päbst Felicitas,
Burk Janina,
Pfarrer Christiane,
Staszyk Carsten
Publication year - 2017
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.74
Subject(s) - mesenchymal stem cell , stromal cell , bone marrow , medicine , cancer research , biology , pathology
Allogeneic equine multipotent mesenchymal stromal cells ( eMSC s) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSC s might be the bone marrow from euthanized horses. The purpose of this study was to compare in vitro characteristics of equine bone marrow derived eMSC ( eBM ‐ MSC s) from euthanized horses (eut‐ MSC s) and from narcotized horses (nar‐MSCs). Eut‐ MSC s and nar‐ MSC s showed typical eMSC marker profiles (positive: CD 44, CD 90; negative: CD 11a/ CD 18 and MHCII ) and possessed tri‐lineage differentiation characteristics. Although CD 105 and MHCI expression varied, no differences were detected between eut‐ MSC s and nar‐ MSC s. Proliferation characteristics did not differ between eut‐ MSC s and nar‐ MSC s, but age dependent decrease in proliferation and increase in MHCI expression was detected. These results suggest the possible use of eut‐ MSC s for therapeutic applications and production of commercial available eBM ‐ MSC products.