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Toxoplasmosis is a worldwide zoonosis caused by the parasitic protozoan  Toxoplasma gondii . It can infect all warm‐blooded vertebrate species and causes abortions and birth defects in pregnant women and pregnant ewes. The objective of this study was to estimate the prevalence of infection with  T. gondii  in sheep meat in the region of Sidi Bouzid (central Tunisia) and Beja (northern Tunisia), the realization of a descriptive study of risk factors and the phylogenetic analyses of  T. gondii . Neck muscle samples were obtained from 174 ewes and ewe lamb slaughtered in Sidi Bouzid and 150 lambs slaughtered in Beja.  DNA  was extracted from the samples using the Wizard ®  genomic DNA purification kit. A nested  PCR  using two pairs of primers ( NN  1 and  NN 2, Tg‐ NP 1 and Tg‐ NP 2) were used to detect infection with  T. gondii , which was then confirmed by sequencing. Eight  T. gondii  amplicons were sequenced (accession number  KT 896498) and deposited in GenBank. The  T. gondii  amplicons showed 97–100% identities with GenBank sequences. A phylogenetic tree was then constructed. The nested  PCR  detected  T. gondii   DNA  in 31% of animals tested in Sidi Bouzid and 32% of lambs tested in Beja. No significant difference in the prevalence of  T. gondii  infection was established between the two tested regions. In both regions, no significant variation of the infection depending on age, breed and locality was found.

Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia