Open AccessExtract of neem ( Azadirachta indica ) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry
Author(s) -
Ali Edris,
Islam Md. Sadequl,
Hossen Md. Ismail,
Khatun Mst. Minara,
Islam Md. Ariful
Publication year - 2021
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.511
Subject(s) - azadirachta , minimum bactericidal concentration , pathogenic bacteria , minimum inhibitory concentration , salmonella , pasteurella multocida , biology , microbiology and biotechnology , multiple drug resistance , bacteria , veterinary medicine , antibiotics , horticulture , medicine , genetics
The aim of the study was to determine the efficacy of neem leaf extract against multidrug resistant (MDR) pathogenic bacteria. Laboratory stock culture of Pasteurella multocida, Salmonella pullorum, Salmonella gallinarum and Escherichia coli was revived. Antibiogram profiles of these bacteria were determined by disc diffusion method. Ethanolic extract of neem leaf was prepared. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of neem leaf extract (112.5, 100, 50, 25, 12.5, 6.25 and 3.12 mg/ml) against MDR pathogenic bacteria of poultry were determined by double dilution method. The MIC and MBC of the neem leaf extract were 12.5 and 25 mg/ml, respectively for P. multocida , 50 and 100 mg/ml for S. pullorum and S. gallinarum , 100 and 112.5 mg/ml for E. coli . Neem leaf extracts exhibited bactericidal effect against MDR pathogenic bacteria of poultry.