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Equid herpesviruses ( EHV s) threaten equine health and can cause significant economic losses to the equine industry worldwide. Different equid herpesviruses,  EHV ‐1,  EHV ‐2,  EHV ‐4 and  EHV 5 are regularly detected among horse populations. In Egypt, monitoring is sporadic but  EHV ‐1 or  EHV ‐4 have been reported to circulate in the horse population. However, there is a lack of reports related to infection and health status of horses, likely due to the absence of regular diagnostic procedures. In the current study, the circulation of four infectious equid herpesviruses ( EHV ‐1,  EHV ‐2,  EHV ‐4 and  EHV ‐5) among different Arabian horse populations and donkeys residing the same farm was monitored. Different samples were collected and  DNA  was extracted and subjected to quantitative (q)‐ PCR  to detect the four equid herpesviruses using specific primers and probes. Antibody titres against  EHV ‐1 and  EHV ‐4 were tested using virus neutralization test and type‐specific  ELISA . The results showed that  EHV ‐1,  EHV ‐2,  EHV ‐4 and  EHV ‐5 are endemic and can be a continuous threat for horses in the absence of vaccination programs and frequent virus reactivation. There is an urgent need for introduction of active regular surveillance measures to investigate the presence of different equid herpesviruses, and other equine viral pathogens, in various horse populations around Egypt and to establish a standardized cataloguing of equine health status.

Detection of equid herpesviruses among different Arabian horse populations in Egypt