Open AccessmRNA expression profiling in cotyledons reveals significant up‐regulation of the two bovine pregnancy‐associated glycoprotein genes bo PAG ‐8 and bo PAG ‐11 in early gestation
Author(s) -
Wiedemann Isabel,
Krebs Tony,
Momberg Niklas,
Knorr Christoph,
Tetens Jens
Publication year - 2018
Publication title -
veterinary medicine and science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.485
H-Index - 11
ISSN - 2053-1095
DOI - 10.1002/vms3.123
Subject(s) - glycoprotein , trophoblast , messenger rna , biology , gene expression , glycosylation , gene , microbiology and biotechnology , placenta , pregnancy , biochemistry , genetics , fetus
The multigene family of pregnancy‐associated glycoproteins ( PAG s) belongs to a group of aspartic proteases that are exclusively expressed by trophoblast cells in the placenta of even‐toed ungulates. In Bovidae , 22 different PAG genes (bo PAG s) with a wide range of temporal and spatial expression‐ and glycosylation patterns have been reported to date. In this study we describe the mRNA expression patterns using real‐time quantitative PCR ( qPCR ) for selected modern (bo PAG ‐1, ‐9, ‐21) and ancient bovine PAG s (bo PAG ‐2, ‐8, ‐10, ‐11, ‐12) in cotyledonary tissue. The highest mean expression was detected in bo PAG ‐8 and lowest in bo PAG ‐10 ( P < 0.05). Furthermore, bo PAG ‐8 and ‐11 were significantly greater expressed in early gestation compared with later pregnancy stages. The characterization of bo PAG mRNA ‐expression levels gives important insights for further protein analyses which will be valuable information for the development of new pregnancy detection systems.