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Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues
Author(s) -
Weidenhamer Nathan K.,
Moore Dusty L.,
Lobo Fluvio L.,
Klair Nathaniel T.,
Tranquillo Robert T.
Publication year - 2015
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1974
Subject(s) - decellularization , extracellular matrix , mesenchymal stem cell , ascorbic acid , tissue engineering , microbiology and biotechnology , regenerative medicine , chemistry , matrix (chemical analysis) , myofibroblast , hyaluronic acid , fibroblast , scaffold , biomedical engineering , stem cell , in vitro , anatomy , biochemistry , biology , pathology , fibrosis , medicine , food science , chromatography
The variables that influence the in vitro recellularization potential of decellularized engineered tissues, such as cell culture conditions and scaffold alignment, have yet to be explored. The goal of this work was to explore the influence of insulin and ascorbic acid and extracellular matrix (ECM) alignment on the recellularization of decellularized engineered tissue by human mesenchymal stem cells (hMSCs). Aligned and non‐aligned tissues were created by specifying the geometry and associated mechanical constraints to fibroblast‐mediated fibrin gel contraction and remodelling using circular and C‐shaped moulds. Decellularized tissues (matrices) of the same alignment were created by decellularization with detergents. Ascorbic acid promoted the invasion of hMSCs into the matrices due to a stimulated increase in motility and proliferation. Invasion correlated with hyaluronic acid secretion, α ‐smooth muscle actin expression and decreased matrix thickness. Furthermore, hMSCs invasion into aligned and non‐aligned matrices was not different, although there was a difference in cell orientation. Finally, we show that hMSCs on the matrix surface appear to differentiate toward a smooth muscle cell or myofibroblast phenotype with ascorbic acid treatment. These results inform the strategy of recellularizing decellularized engineered tissue with hMSCs. © 2015 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd.