Open AccessInducible Cassette Exchange: A Rapid and Efficient System Enabling Conditional Gene Expression in Embryonic Stem and Primary Cells
Author(s) -
Iacovino Michelina,
Bosnakovski Darko,
Fey Holger,
Rux Danielle,
Bajwa Gagan,
Mahen Elisabeth,
Mitanoska Ana,
Xu Zhaohui,
Kyba Michael
Publication year - 2011
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.715
Subject(s) - biology , embryonic stem cell , stem cell , gene , microbiology and biotechnology , gene expression , primary (astronomy) , genetics , computational biology , physics , astronomy
Abstract Genetic modification is critically enabling for studies addressing specification and maintenance of cell fate; however, methods for engineering modifications are inefficient. We demonstrate a rapid and efficient recombination system in which an inducible, floxed cre allele replaces itself with an incoming transgene. We target this inducible cassette exchange (ICE) allele to the ( HPRT ) locus and demonstrate recombination in murine embryonic stem cells (ESCs) and primary cells from derivative ICE mice. Using lentivectors, we demonstrate recombination at a randomly integrated ICE locus in human ESCs. To illustrate the utility of this system, we insert the myogenic regulator, Myf5 , into the ICE locus in each platform. This enables efficient directed differentiation of mouse and human ESCs into skeletal muscle and conditional myogenic transdetermination of primary cells cultured in vitro. This versatile tool is thus well suited to gain‐of‐function studies probing gene function in the specification and reprogramming of cell fate. S TEM C ELLS 2011;29:1580–1588