Open AccessStrontium Enhances Osteogenic Differentiation of Mesenchymal Stem Cells and In Vivo Bone Formation by Activating Wnt/Catenin Signaling
Author(s) -
Yang Fan,
Yang Dazhi,
Tu Jie,
Zheng Qixin,
Cai Lintao,
Wang Liping
Publication year - 2011
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.646
Subject(s) - strontium ranelate , mesenchymal stem cell , wnt signaling pathway , osteoblast , microbiology and biotechnology , in vivo , osteoclast , bone resorption , strontium , stem cell , biology , materials science , chemistry , in vitro , endocrinology , signal transduction , osteoporosis , biochemistry , organic chemistry
Strontium ranelate is a newly approved drug that can reduce the risk of vertebral fracture, which is attributed to its dual function in increasing the bone formation and decreasing the bone resorption. Strontium‐containing hydroxyapatite was also demonstrated to stimulate the osteoblast activity and inhibit the osteoclast activity. However, the molecular mechanisms of strontium underlying such beneficial effects were still not fully understood. In this study, we investigated the effects of strontium on the osteogenic differentiation of human mesenchymal stem cells (MSCs) and its related mechanism; its osteogenic potential was also evaluated using a calvarial defect model in rats. We found that strontium could enhance the osteogenic differentiation of the MSCs, with upregulated extracellular matrix (ECM) gene expression and activated Wnt/β‐catenin pathway. After transplanting the collagen‐strontium‐substituted hydroxyapatite scaffold into the bone defect region, histology and computed tomography scanning revealed that in vivo bone formation was significantly enhanced; the quantity of mature and remodeled bone substantially increased and ECM accumulated. Interestingly, strontium induced an increase of β‐catenin expression in newly formed bone area. In this study, we showed for the first time that strontium could stimulate the β‐catenin expression in vitro and in vivo, which might contribute to the enhanced osteogenic differentiation of MSCs and in vivo bone formation. STEM CELLS 2011;29:981–991