Open AccessEpigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates
Author(s) -
Sparman Michelle,
Dighe Vikas,
Sritanaudomchai Hathaitip,
Ma Hong,
Ramsey Cathy,
Pedersen Darlene,
Clepper Lisa,
Nighot Prashant,
Wolf Don,
Hennebold Jon,
Mitalipov Shoukhrat
Publication year - 2009
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.60
Subject(s) - reprogramming , biology , somatic cell , microbiology and biotechnology , somatic cell nuclear transfer , epigenetics , induced pluripotent stem cell , embryonic stem cell , stem cell , cell potency , oocyte , dna methylation , genetics , blastocyst , cell , embryo , gene expression , embryogenesis , gene
We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor cells. Two ES cell lines were isolated using adult female rhesus macaque skin fibroblasts as nuclear donors and oocytes retrieved from one female, following a single controlled ovarian stimulation. In addition to routine pluripotency tests involving in vitro and in vivo differentiation into various somatic cell types, primate ES cells derived from reprogrammed somatic cells were also capable of contributing to cells expressing markers of germ cells. Moreover, imprinted gene expression, methylation, telomere length, and X‐inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte‐specific factors. S TEM C ELLS 2009;27:1255–1264