Open AccessBrief Report: Combined Chemical Treatment Enables Oct4 ‐Induced Reprogramming from Mouse Embryonic Fibroblasts
Author(s) -
Yuan Xu,
Wan Haifeng,
Zhao Xiaoyang,
Zhu Saiyong,
Zhou Qi,
Ding Sheng
Publication year - 2011
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.594
Subject(s) - reprogramming , biology , sox2 , klf4 , induced pluripotent stem cell , embryonic stem cell , microbiology and biotechnology , homeobox protein nanog , transcription factor , somatic cell , stem cell , genetics , cell , gene
It has been established that exogenous expression of four transcription factors ( Oct4 , Klf4 , Sox2 , and c‐Myc ) can reprogram mammalian somatic cells to pluripotent states. Further studies demonstrated that such induced pluripotent stem cells (iPSCs) could be generated with fewer exogenous transcription factors, facilitated by endogenous expression of reprogramming factors and/or synthetic small molecules. Here, we reported identification of a new small molecule, a protein arginine methyltransferase inhibitor AMI‐5, which enabled Oct4 ‐induced reprogramming of mouse embryonic fibroblasts in combination with transforming growth factor (TGF)‐β inhibitor A‐83‐01. The Oct4 ‐induced iPSCs were shown similar to mouse embryonic stem cells with respect to typical pluripotency criteria. More importantly, they were shown to give rise to liveborn pups through tetraploid complementation assays, demonstrating the high quality of full reprogramming induced by this condition. Furthermore, this study suggests that regulation of protein arginine methylation might be involved in the reprogramming process. S TEM C ELLS 2011;29:549–553