Open AccessComprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein‐1 (CD71) as a Negative Selection Marker for Human Neuronal Cells
Author(s) -
Me Vishal,
Thomas Ria,
Elgueta Claudio,
Horl Marcus,
Osborn Teresia,
Hallett Penny J.,
Bartos Marlene,
Isacson Ole,
Pruszak Jan
Publication year - 2019
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.3057
Subject(s) - biology , transferrin receptor , neural stem cell , cluster of differentiation , induced pluripotent stem cell , stem cell , microbiology and biotechnology , neural cell , cellular differentiation , cell , flow cytometry , lineage markers , embryonic stem cell , genetics , progenitor cell , gene
Cell state‐, developmental stage‐, and lineage‐specific combinatorial expression of cluster of differentiation (CD) molecules enables the identification of cellular subsets via multicolor flow cytometry. We describe an exhaustive characterization of neural cell types by surface antigens, exploiting human pluripotent stem cell‐derived neural cell systems. Using multiwell screening approaches followed by detailed validation of expression patterns and dynamics, we exemplify a strategy for resolving cellular heterogeneity in stem cell paradigms. In addition to providing a catalog of surface antigens expressed in the neural lineage, we identified the transferrin receptor‐1 (CD71) to be differentially expressed in neural stem cells and differentiated neurons. In this context, we describe a role for N‐Myc proto‐oncogene (MYCN) in maintaining CD71 expression in proliferating neural cells. We report that in vitro human stem cell‐derived neurons lack CD71 surface expression and that the observed differential expression can be used to identify and enrich CD71 − neuronal derivatives from heterogeneous cultures. Stem Cells 2019;37:1293–1306