Open AccessTransient MicroRNA Expression Enhances Myogenic Potential of Mouse Embryonic Stem Cells
Author(s) -
Bem Joanna,
Grabowska Iwona,
Daniszewski Maciej,
Zawada Dorota,
Czerwinska Areta M.,
Bugajski Lukasz,
Piwocka Katarzyna,
Fogtman Anna,
Ciemerych Maria A.
Publication year - 2018
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.2772
Subject(s) - biology , myogenesis , embryoid body , microbiology and biotechnology , embryonic stem cell , myf5 , cellular differentiation , microrna , pax3 , stem cell , myod , induced pluripotent stem cell , genetics , myocyte , gene , transcription factor
MicroRNAs (miRNAs) are known regulators of various cellular processes, including pluripotency and differentiation of embryonic stem cells (ESCs). We analyzed differentiation of two ESC lines—D3 and B8, and observed significant differences in the expression of miRNAs and genes involved in pluripotency and differentiation. We also examined if transient miRNA overexpression could serve as a sufficient impulse modulating differentiation of mouse ESCs. ESCs were transfected with miRNA Mimics and differentiated in embryoid bodies and embryoid body outgrowths. miRNAs involved in differentiation of mesodermal lineages, such as miR145 and miR181, as well as miRNAs regulating myogenesis (MyomiRs)—miR1, miR133a, miR133b, and miR206 were tested. Using such approach, we proved that transient overexpression of molecules selected by us modulated differentiation of mouse ESCs. Increase in miR145 levels upregulated Pax3 , Pax7 , Myod1, Myog, and MyHC2, while miR181 triggered the expression of such crucial myogenic factors as Myf5 and MyHC2 . As a result, the ability of ESCs to initiate myogenic differentiation and form myotubes was enhanced. Premature expression of MyomiRs had, however, an adverse effect on myogenic differentiation of ESCs. S tem C ells 2018;36:655–670