Open AccessGeneration of Human‐Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2
Author(s) -
Li Wenlin,
Zhou HongYan,
Abujarour Ramzey,
Zhu Saiyong,
Young Joo Jin,
Lin Tongxiang,
Hao Ergeng,
Schöler Hans R.,
Hayek Alberto,
Ding Sheng
Publication year - 2009
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.240
Subject(s) - biology , induced pluripotent stem cell , sox2 , stem cell , microbiology and biotechnology , human induced pluripotent stem cells , genetics , embryonic stem cell , gene
Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK‐3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named tranylcypromine), an inhibitor of lysine‐specific demethylase 1, CHIR99021 can cause the reprogramming of human primary keratinocyte transduced with the two factors, Oct4 and Klf4. To our knowledge, this is the first time that human iPS cells have been generated from somatic cells without exogenous Sox2 expression. Our studies suggest that the GSK‐3 inhibitor might have a general application to replace transcription factors in both mouse and human reprogramming. S TEM C ELLS 2009;27:2992–3000