Open AccessCorrelation Between IL‐3 Receptor Expression and Growth Potential of Human CD34 + Hematopoietic Cells from Different Tissues
Author(s) -
Huang Shiang,
Chen Zhang,
Yu Ji Feng,
Young Dennis,
Bashey Asad,
Ho Anthony D.,
Law Ping
Publication year - 1999
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.170265
Subject(s) - biology , cd34 , haematopoiesis , cord blood , progenitor cell , stem cell , microbiology and biotechnology , bone marrow , stem cell factor , interleukin 3 , cd38 , immunology , antigen presenting cell , t cell , immune system
CD123 (α‐subunit of IL‐3 receptor) expression on primitive and committed human hematopoietic cells was studied by multicolor sorting and single‐cell culture. The sources of cells included fetal liver (FLV), fetal bone marrow, umbilical cord blood, adult bone marrow and mobilized peripheral blood. Three subsets of CD34 + cells were defined by the levels of surface CD123: CD123 negative , CD123 low , and CD123 bright . Coexpression of lineage markers showed that a majority of CD34 + CD123 bright cells were myeloid and B‐lymphoid progenitors, while erythroid progenitors were mainly in the CD34 + CD123 negative subset. The CD34 + CD123 low subset contained a heterogeneous distribution of early and committed progenitor cells. Single CD34 + cells from the CD123 subsets were cultured in a cytokine cocktail of stem cell factor, interleukin 3 (IL‐3), IL‐6, GM‐CSF, erythropoietin, insulin‐like growth factor‐1, and basic fibroblast growth factor. After 14 days of incubation, a higher cloning efficiency (CE) was observed in the CD34 + CD123 negative and CD34 + CD123 low fractions (37 ± 23% and 44 ± 23%, respectively) than in the CD34 + CD123 bright fraction (15 ± 21%). Using previously published criteria that colonies containing dispersed, translucent cells (dispersed growth pattern, DGP) were derived from primitive cells and that colonies composed solely of clusters were from committed cells, early precursors were distributed evenly in the CD34 + CD123 negative and CD34 + CD123 low subsets. When CD38 and CD90 (Thy‐1) were used for further characterization of CD34 + cells from FLV, CE increased from 37 ± 23% in CD123 negative to 70 ± 19% in CD123 negative CD38 − and from 44 ± 23% in CD123 low to 66 ± 19% in CD123 low CD38 − . No significant increase in CE or DGP progenitors was observed when CD34 + cells were sorted by CD90 and CD123. We concluded that: A) high levels of CD123 were expressed on B‐lymphoid and myeloid progenitors; B) early erythroid progenitors had little or no surface CD123, and C) primitive hematopoietic cells are characterized by CD123 negative/low expression.