Open AccessLgr4 is a key regulator of prostate development and prostate stem cell differentiation
Author(s) -
Luo Weijia,
Rodriguez Melissa,
Valdez Joseph M.,
Zhu Xinglei,
Tan Kunrong,
Li Dali,
Siwko Stefan,
Xin Li,
Liu Mingyao
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1484
Subject(s) - biology , wnt signaling pathway , stem cell , prostate , microbiology and biotechnology , sonic hedgehog , endocrinology , cellular differentiation , hedgehog , medicine , hedgehog signaling pathway , lncap , cancer research , signal transduction , biochemistry , cancer , gene , genetics
A bstract Mechanisms modulating prostate cell fate determination remain unexplored. The leucine‐rich repeat containing G‐protein‐coupled receptors (Lgr) have been identified as important stem cell markers in various tissues. Here, we investigated the roles of Lgr4/Gpr48 in prostate stem cells (PSCs) and development. Lgr4 was ubiquitously expressed during early prostate development prior to lineage specification, with adult expression restricted to a few basal cells (principally Lin − Sca1 + CD49f + ). Lgr4 − / − mice had compromised branching morphogenesis and delayed epithelial differentiation, leading to decreased prostate size and impaired luminal cell function. In vitro prostate sphere culture revealed that Lgr4 − / − Lin − /Sca1 + /CD49f + cells failed to generate p63 low cells, indicating a differentiation deficiency. Furthermore, Lgr4 ablation arrested PSC differentiation of in vivo kidney capsule prostate grafts, suggesting that Lgr4 modulates PSC properties independent of hormonal and mesenchymal effects. Analysis of neonatal prostates and prostate spheres revealed a decrease in Wnt, Sonic Hedgehog, and Notch1 expression in Lgr4 − / − cells. Lgr4 loss blocked differentiation of prostate sphere p63 hi cells to p63 low . Treatment with exogenous Sonic Hedgehog partially restored the differentiation of p63 hi cells in Lgr4 − / − spheres. Taken together, our data revealed the roles of Lgr4 in early prostate development and in stem cell differentiation through regulation of the Wnt, Notch, and Sonic Hedgehog signaling pathways. S tem C ells 2013;31:2492–2505