Open AccessAstrocytic Adenosine 5′‐Triphosphate Release Regulates the Proliferation of Neural Stem Cells in the Adult Hippocampus
Author(s) -
Cao Xiong,
Li LiangPing,
Qin XiHe,
Li ShuJi,
Zhang Meng,
Wang Qian,
Hu HongHai,
Fang YingYing,
Gao YuBo,
Li XiaoWen,
Sun LiRong,
Xiong WenChao,
Gao TianMing,
Zhu XinHong
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1408
Subject(s) - neurogenesis , biology , neural stem cell , astrocyte , purinergic receptor , microbiology and biotechnology , hippocampus , purinergic signalling , hippocampal formation , adenosine triphosphate , adenosine , stem cell , neuroscience , adenosine receptor , receptor , endocrinology , biochemistry , central nervous system , extracellular , agonist
Astrocytes are key components of the niche for neural stem cells (NSCs) in the adult hippocampus and play a vital role in regulating NSC proliferation and differentiation. However, the exact molecular mechanisms by which astrocytes modulate NSC proliferation have not been identified. Here, we identified adenosine 5′‐triphosphate (ATP) as a proliferative factor required for astrocyte‐mediated proliferation of NSCs in the adult hippocampus. Our results indicate that ATP is necessary and sufficient for astrocytes to promote NSC proliferation in vitro. The lack of inositol 1,4,5‐trisphosphate receptor type 2 and transgenic blockage of vesicular gliotransmission induced deficient ATP release from astrocytes. This deficiency led to a dysfunction in NSC proliferation that could be rescued via the administration of exogenous ATP. Moreover, P2Y1‐mediated purinergic signaling is involved in the astrocyte promotion of NSC proliferation. As adult hippocampal neurogenesis is potentially involved in major mood disorder, our results might offer mechanistic insights into this disease. S TEM C ells 2013;31:1633–1643