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Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes  CD24  and  CD44  are differentially expressed across  LGR5  positive “active” stem cells as well as  HOPX  positive “facultative” stem cells. Fluorescence‐activated cell sorting enables differential enrichment of  LGR5  (CD24−/CD44+) and  HOPX  (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease. S TEM  C ells   2013;31:2024‐2030

Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells