Open AccessBrief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells
Author(s) -
Gracz Adam D.,
Fuller Megan K.,
Wang Fengchao,
Li Linheng,
Stelzner Matthias,
Dunn James C.Y.,
Martin Martin G.,
Magness Scott T.
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1391
Subject(s) - biology , stem cell , cd44 , lgr5 , cd24 , microbiology and biotechnology , stem cell marker , cell sorting , cluster of differentiation , immunology , cell , cancer stem cell , flow cytometry , genetics
Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes CD24 and CD44 are differentially expressed across LGR5 positive “active” stem cells as well as HOPX positive “facultative” stem cells. Fluorescence‐activated cell sorting enables differential enrichment of LGR5 (CD24−/CD44+) and HOPX (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease. S TEM C ells 2013;31:2024‐2030