Open AccessConcise Review: Maturation Phases of Human Pluripotent Stem Cell‐Derived Cardiomyocytes
Author(s) -
Robertson Claire,
Tran David D.,
George Steven C.
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1331
Subject(s) - biology , induced pluripotent stem cell , phenotype , microbiology and biotechnology , electrophysiology , stem cell , intracellular , fetus , cellular differentiation , cell physiology , cell culture , cell , embryonic stem cell , neuroscience , gene , genetics , pregnancy
Abstract Human pluripotent stem cell‐derived cardiomyocytes (hPS‐CM) may offer a number of advantages over previous cardiac models, however, questions of their immaturity complicate their adoption as a new in vitro model. hPS‐CM differ from adult cardiomyocytes with respect to structure, proliferation, metabolism and electrophysiology, better approximating fetal cardiomyocytes. Time in culture appears to significantly impact phenotype, leading to what can be referred to as early and late hPS‐CM. This work surveys the phenotype of hPS‐CM, including structure, bioenergetics, sensitivity to damage, gene expression, and electrophysiology, including action potential, ion channels, and intracellular calcium stores, while contrasting fetal and adult CM with hPS‐CM at early and late time points after onset of differentiation. S TEM C ELLS 2013;31:829–837