Open AccessInjectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells
Author(s) -
Yamada Yoichi,
Nakamura Sayaka,
Ito Kenji,
Umemura Eri,
Hara Kenji,
Nagasaka Tetsuro,
Abe Akihiro,
Baba Shunsuke,
Furuichi Yasushi,
Izumi Yuichi,
Klein Ophir D.,
Wakabayashi Toshihiko
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1300
Subject(s) - mesenchymal stem cell , stem cell , tissue engineering , stem cell therapy , bone tissue , transplantation , bone resorption , biology , platelet rich plasma , medicine , surgery , pathology , biomedical engineering , platelet , microbiology and biotechnology
Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectable tissue‐engineered bone (TEB) composed of mesenchymal stem cells (MSCs) and platelet‐rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out large‐scale clinical studies in patients with long‐term follow‐up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months were significantly increased over the preoperation baseline ( p < .001) and reached levels equivalent to that of native bone. No significant bone resorption occurred during long‐term follow‐up. Injectable TEB restored masticatory function in patients. This novel clinical approach represents an effective therapeutic utilization of bone tissue engineering. S TEM C ELLS 2013;31:572–580