Open AccessAmnion membrane hydrogel and amnion membrane powder accelerate wound healing in a full thickness porcine skin wound model
Author(s) -
Murphy Sean V.,
Skardal Aleksander,
Nelson Ronald A.,
Sun Khiry,
Reid Tanya,
Clouse Cara,
Kock Nancy D.,
Jackson John,
Soker Shay,
Atala Anthony
Publication year - 2020
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.19-0101
Subject(s) - amnion , wound healing , dermis , human skin , biomedical engineering , medicine , biology , surgery , pathology , fetus , pregnancy , genetics
There is a need for effective wound treatments that retain the bioactivity of a cellular treatment, but without the high costs and complexities associated with manufacturing, storing, and applying living biological products. Previously, we developed an amnion membrane‐derived hydrogel and evaluated its wound healing properties using a mouse wound model. In this study, we used a full thickness porcine skin wound model to evaluate the wound‐healing efficacy of the amnion hydrogel and a less‐processed amnion product comprising a lyophilized amnion membrane powder. These products were compared with commercially available amnion and nonamnion wound healing products. We found that the amnion hydrogel and amnion powder treatments demonstrated significant and rapid wound healing, driven primarily by new epithelialization versus closure by contraction. Histological analysis demonstrated that these treatments promote the formation of a mature epidermis and dermis with similar composition to healthy skin. The positive skin regenerative outcomes using amnion hydrogel and amnion powder treatments in a large animal model further demonstrate their potential translational value for human wound treatments.