Open AccessA Comprehensive Analysis of Protocols for Deriving Dopaminergic Neurons from Human Pluripotent Stem Cells
Author(s) -
Marton Rebecca M.,
Ioannidis John P. A.
Publication year - 2019
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.18-0088
Subject(s) - induced pluripotent stem cell , novelty , dopaminergic , stem cell , embryonic stem cell , neuroscience , human induced pluripotent stem cells , biology , protocol (science) , computer science , microbiology and biotechnology , medicine , dopamine , psychology , pathology , genetics , social psychology , gene , alternative medicine
The potential applications of human embryonic and induced pluripotent stem cells has led to immense interest in developing new protocols to differentiate specific cell types or modifying existing protocols. To investigate to what extent and why new protocols for the same cell types are developed and adopted, we systematically evaluated 158 publications (2004‐2017) that differentiated human stem cells into dopaminergic neurons. We categorized each article by degree of novelty and recorded motivations for protocol development. 74 novel or modified protocols were developed. Most (65%) were not used again in subsequent studies. Diverse motivations were recorded and performance of new methods was assessed with substantially different approaches across studies. There was improvement over time in yield of neuron production, but not in yield of dopaminergic neurons or time required for getting neurons. Standardized reporting of performance metrics may help rational choice of the best methods. Stem Cells Translational Medicine 2019;8:366–374