Open AccessA multicenter study to evaluate automated platelet aggregometry on Sysmex CS‐series coagulation analyzers—preliminary findings
Author(s) -
Platton Sean,
McCormick Áine,
Bukht Musfira,
Gurney David,
Holding Ian,
Moore Gary W.
Publication year - 2018
Publication title -
research and practice in thrombosis and haemostasis
Language(s) - English
Resource type - Journals
ISSN - 2475-0379
DOI - 10.1002/rth2.12140
Subject(s) - ristocetin , chemistry , arachidonic acid , whole blood , chromatography , epinephrine , platelet , reference range , platelet aggregation , medicine , biochemistry , enzyme
Background and Objectives Investigations of platelet function by light transmission aggregometry ( LTA ) using a dedicated aggregometer is time consuming and labor intensive. This multicenter study evaluated an automated LTA method using a coagulation analyzer to establish reference ranges and ideal testing regimen. Methods Sysmex CS ‐2x00 series analyzers were used to measure aggregation using a range of agonists and concentrations: ADP (1‐20 μM); arachidonic acid (0.5‐1.5 mM ); collagen (1.25‐5 μg/ mL ); ristocetin (0.5‐1.5 g/L); epinephrine (5‐10 μM); TRAP (1‐20 μM); U46619 (1 μM); and saline. Maximum and final aggregation, disaggregation, slope, and acquisition time were compared for each. Results For 42 normal subjects there was no significant difference in aggregation parameters for: 10 μM and 20 μm ADP ; 2 and 2.5 μM ADP ; 1 and 1.5 mM arachidonic acid; 2.5 and 5 μg/ mL collagen; 1 and 1.25 μg/ mL collagen; 1.25 and 1.5 g/L ristocetin; 5 and 10 μM epinephrine; 5 and 10 μM or 20 μM TRAP . Maximum aggregation was reached by 300 seconds with 20 and 10 μM ADP , 1 μM U46619, 1 and 1.25 μg/ mL collagen, 1.5 g/L ristocetin and 5, 10, and 20 μM TRAP : all others agonists required 600s. Conclusions A standard panel of agonists can be used on the Sysmex CS ‐2x00 series analyzers: ADP (10, 5, 2.5, and 1.25 μM); 1 mM arachidonic acid; 1 μM U46619; 2.5 and 1.25 μg/ mL collagen; 1.25 and 0.5 g/L ristocetin; 5 μM epinephrine; 5 and 10 μM TRAP ; and saline. Aggregation should be observed for 600 seconds for all agonists except TRAP and U46619, which require 300 seconds. If further studies confirm these concentrations detect platelet disorders then Sysmex CS ‐series analyzers could replace dedicated aggregometers, or perform LTA where it is currently not available.