Open AccessLoss of high‐mobility group N5 contributes to the promotion of human endometrial stromal cell decidualization
Author(s) -
Kimura Machiko,
Kajihara Takeshi,
Mizuno Yumi,
Sato Tomomi,
Ishihara Osamu
Publication year - 2018
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1002/rmb2.12226
Subject(s) - decidualization , stromal cell , chemistry , decidua , histone , microrna , medicine , endocrinology , microbiology and biotechnology , biology , gene , placenta , biochemistry , fetus , pregnancy , genetics
Purpose High‐mobility group N ( HMGN ) proteins are the only non‐histone proteins that specifically bind within the nucleosome between core histones and DNA . Among them, HMGN 5 is one of the candidates that could participate in mouse endometrial decidualization; however, the specific role of HMGN 5 remains to be clarified in human endometrial stromal cells ( HESC s). Methods Primary HESC s were isolated from hysterectomy specimens and incubated with or without 8‐bromo‐cyclic adenosine monophosphate (8‐br‐ cAMP ) and medroxyprogesterone acetate ( MPA ). Results We demonstrated that HMGN 5 expression in decidualized HESC s stimulated by 8‐br‐ cAMP and MPA decreased significantly. The inhibition of HMGN 5 expression by small interfering RNA (si RNA ) induced the major decidual marker genes expression, including IGFBP 1 (insulin‐like growth factor binding protein 1) and PRL (prolactin). In addition, micro RNA ‐542‐3p (miR‐542‐3p), which was identified as a regulatory mi RNA of IGFBP 1 during decidualization, was significantly suppressed by HMGN 5 si RNA . However, the expression of HMGN 5 was not alternated by miR‐542‐3p overexpression. Conclusions These findings suggest that the down‐regulation of HMGN 5 plays a role in the promotion of human endometrial stromal decidualization and acts upstream of miR‐542‐3p.