Open AccessAssociation of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
Author(s) -
Inoue Taketo,
Taguchi Sayumi,
Hirao Kayoko,
Tsujimoto Yoshiko,
Yamamoto Shuji,
Uemura Mikiko,
Miyazaki Kazunori,
Yamashita Yoshiki
Publication year - 2018
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1002/rmb2.12102
Subject(s) - intracytoplasmic sperm injection , follicular fluid , andrology , human fertilization , oocyte , blastocyst , agonist , chemistry , follicular phase , gonadotropin , medicine , endocrinology , hormone , in vitro fertilisation , biology , receptor , anatomy , embryo , embryogenesis , microbiology and biotechnology , biochemistry , gene
Purpose The authors previously revealed the association of the follicular fluid ( FF ) volume with oolemma stretchability following the gonadotropin‐releasing hormone (Gn RH ) antagonist protocol during intracytoplasmic sperm injection ( ICSI ). However, the impact of the Gn RH agonist protocol on oolemma stretchability remains unclear. Methods Data that were obtained from 74 ICSI cycles were reviewed retrospectively. Controlled ovarian stimulation was performed in accordance with the short Gn RH agonist protocol. Each follicle was individually aspirated and assigned to one of six groups, according to the FF volume. The oolemma stretchability during ICSI was evaluated by using a mechanical stimulus for oolemma penetration; that is, oolemma penetration with or without aspiration (high vs low stretchability, respectively). Results The incidence of low oolemma stretchability was significantly higher in the <1.0 mL group than that in the ≥1.0 mL group. The normal fertilization rate was significantly lower in the <1.0 mL group than that in the 2.0‐<3.0 mL group. The rate of blastocyst development was lower in the <1.0 mL group than that in the 3.0‐<4.0 mL group. Conclusion The FF volume potentially was associated with metaphase II oolemma stretchability, fertilization, and blastocyst development.