Open AccessMeiotic spindle size is a strong indicator of human oocyte quality
Author(s) -
Tomari Hiroyuki,
Honjo Ko,
Kunitake Katsuko,
Aramaki Natsumi,
Kuhara Saori,
Hidaka Naomi,
Nishimura Kayoko,
Nagata Yumi,
Horiuchi Toshitaka
Publication year - 2018
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1002/rmb2.12100
Subject(s) - intracytoplasmic sperm injection , blastocyst , meiosis , andrology , oocyte , human fertilization , embryo , gynecology , biology , in vitro fertilisation , embryogenesis , medicine , anatomy , genetics , gene
Abstract Purpose To investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection ( ICSI ). Methods Analyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40 years undergoing ICSI cycles. The meiotic spindle was imaged by using PolScope before ICSI . The oocytes were classified into three groups, according to spindle size: group A (<90 μm 2 ), group B (90‐120 μm 2 ), and group C (>120 μm 2 ). Results Overall, 389 (29.9%) oocytes were classified into group A, 662 (50.8%) into group B, and 251 (19.3%) into group C. The fertilization rate of the group B oocytes was significantly higher than for the A and C oocytes. The blastocyst formation rate in group B was significantly higher than in group A. In addition, the pregnancy rate in group B was significantly higher than in the other two groups. Conclusion The oocytes with a spindle size of 90‐120 μm 2 showed higher fertilization, blastocyst formation, and clinical pregnancy rates than those with larger or smaller spindles. The measurement of the meiotic spindle size thus has a positive predictive value for identifying human embryo developmental potential clinically.