Premium
Direct analysis of pharmaceutical compounds in human plasma with chromatographic resolution using an alkyl‐bonded silica rod column
Author(s) -
Plumb Robert,
Dear Gordon,
Mallett David,
Ayrton John
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.329
Subject(s) - chemistry , chromatography , monolithic hplc column , plasma , human plasma , resolution (logic) , alkyl , column (typography) , analytical chemistry (journal) , detection limit , high performance liquid chromatography , organic chemistry , physics , structural engineering , quantum mechanics , connection (principal bundle) , artificial intelligence , computer science , engineering
Abstract Monolithic columns have been successfully used with steep gradient and high flow rates for the direct analysis of a candidate pharmaceutical compound in human plasma. The monolithic columns showed excellent robustness with nearly 300 20‐µL injections of plasma (diluted 1:1 with water) being made onto one column without significant deterioration in performance. The system gave excellent sensitivity with a limit of quantification of 5 ng/mL being achieved. Unlike previous methods of direct analysis the monolithic columns showed excellent resolution even after nearly 300 plasma injections. The column performance was measured before and after the analysis of the plasma samples. Copyright © 2001 John Wiley & Sons, Ltd.