Premium
252 Cf‐plasma desorption and cesium‐ion liquid secondary‐ion mass spectrometric analysis of recombinant proteins
Author(s) -
Tsarbopoulos A.,
Pramanik B. N.,
Reichert P.,
Siegel M. M.,
Nagabhushan T. L.,
Trota P. P.,
Jardine I.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290050208
Subject(s) - chemistry , caesium , ion , desorption , mass spectrometry , plasma , analytical chemistry (journal) , radiochemistry , chromatography , inorganic chemistry , nuclear physics , adsorption , organic chemistry , physics
Abstract The keV Cs + liquid secondary‐ion mass spectrometry (LSIMS) and 252 Cf‐plasma desorption (PD) mass spectra of recombinant proteins in the 10–25 kDa mass range are compared. Both techniques showed comparable mass accuracy and sensitivity, and in the case of LSIMS, remarkably short analysis time. Analysis by the PD/nitrocellulose method demonstrated slightly higher sensitivity and relativity lower dependence on the salt and buffer content of the protein sample.