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PEOPLE: PatiEnt prOstate samPLes for rEsearch, a tissue collection pathway utilizing magnetic resonance imaging data to target tumor and benign tissue in fresh radical prostatectomy specimens
Author(s) -
Heavey Susan,
Costa Helena,
Pye Hayley,
Burt Emma C.,
Jenkinson Sophia,
Lewis GeorginaRose,
BosshardCarter Leticia,
Watson Fran,
Jameson Charles,
Ratynska Marzena,
BenSalha Imen,
Haider Aiman,
Johnston Edward W.,
Feber Andrew,
Shaw Greg,
Sridhar Ashwin,
Nathan Senthil,
Rajan Prabhakar,
Briggs Timothy P.,
Sooriakumaran Prasanna,
Kelly John D.,
Freeman Alex,
Whitaker Hayley C.
Publication year - 2019
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.23782
Subject(s) - prostatectomy , prostate , prostate cancer , medicine , magnetic resonance imaging , ex vivo , sampling (signal processing) , pathology , radiology , in vivo , cancer , biology , computer science , microbiology and biotechnology , filter (signal processing) , computer vision
Background Over 1 million men are diagnosed with prostate cancer each year worldwide, with a wide range of research programs requiring access to patient tissue samples for development of improved diagnoses and treatments. A random sampling of prostate tissue is sufficient for certain research studies; however, there is growing research need to target areas of the aggressive tumor as fresh tissue. Here we set out to develop a new pathway “PEOPLE: PatiEnt prOstate samPLes for rEsearch” to collect high‐quality fresh tissue for research use, using magnetic resonance imaging (MRI) to target areas of tumor and benign tissue. Methods Prostate tissue was sampled following robotic radical prostatectomy, using MRI data to target areas of benign and tumor tissue. Initially, 25 cases were sampled using MRI information from clinical notes. A further 59 cases were sampled using an optimized method that included specific MRI measurements of tumor location along with additional exclusion criteria. All cases were reviewed in batches with detailed clinical and histopathological data recorded. For one subset of samples, DNA was extracted and underwent quality control. Ex vivo culture was carried out using the gelatin sponge method for an additional subset. Results Tumor was successfully fully or partially targeted in 64% of the initial cohort and 70% of the optimized cohort. DNA of high quality and concentration was isolated from 39 tumor samples, and ex vivo culture was successfully carried out in three cases with tissue morphology, proliferation, and apoptosis remaining comparable before and after 72 hours culture. Conclusion Here we report initial data from the PEOPLE pathway; using a method for targeting areas of tumor within prostate samples using MRI. This method operates alongside the standard clinical pathway and minimizes additional input from surgical, radiological, and pathological teams, while preserving surgical margins and diagnostic tissue.