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High performance CCD camera system for digitalisation of 2D DIGE gels
Author(s) -
Strijkstra Annemieke,
Trautwein Kathleen,
Roesler Stefan,
Feenders Christoph,
Danzer Daniel,
Riemenschneider Udo,
Blasius Bernd,
Rabus Ralf
Publication year - 2016
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201500385
Subject(s) - charge coupled device , scanner , laser , optics , laser scanning , physics
An essential step in 2D DIGE‐based analysis of differential proteome profiles is the accurate and sensitive digitalisation of 2D DIGE gels. The performance progress of commercially available charge‐coupled device (CCD) camera‐based systems combined with light emitting diodes (LED) opens up a new possibility for this type of digitalisation. Here, we assessed the performance of a CCD camera system (Intas Advanced 2D Imager) as alternative to a traditionally employed, high‐end laser scanner system (Typhoon 9400) for digitalisation of differential protein profiles from three different environmental bacteria. Overall, the performance of the CCD camera system was comparable to the laser scanner, as evident from very similar protein abundance changes (irrespective of spot position and volume), as well as from linear range and limit of detection.