Open AccessSupramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
Author(s) -
Yue YuXin,
Kong Yong,
Yang Fan,
Zheng Zhe,
Hu XinYue,
Guo DongSheng
Publication year - 2019
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201900316
Subject(s) - chemistry , supramolecular chemistry , pyridoxal , tandem , alkaline phosphatase , fluorescein , phosphate , substrate (aquarium) , combinatorial chemistry , fluorescence , stereochemistry , biochemistry , enzyme , organic chemistry , molecule , biology , materials science , physics , quantum mechanics , composite material , ecology
Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label‐free, continuous, and real‐time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal‐5′‐phosphate (biomarker for several diseases).