Open AccessLabel‐Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host‐Dye Pairs
Author(s) -
Liu YanCen,
Peng Shu,
Angelova Lora,
Nau Werner M.,
Hennig Andreas
Publication year - 2019
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201900299
Subject(s) - lucigenin , phosphatase , fluorescence , chemistry , enzyme , biochemistry , kinase , substrate (aquarium) , phosphorylation , high throughput screening , combinatorial chemistry , biology , ecology , physics , quantum mechanics , superoxide
The combination of the macrocyclic hosts p‐ sulfonatocalix[4]arene and cucurbit[7]uril with the fluorescent dyes lucigenin and berberine affords two label‐free enzyme assays for the detection of kinase and phosphatase activity by fluorescence monitoring. In contrast to established assays, no substrate labeling is required. Since phosphorylation is one of the most important regulatory mechanisms in biological signal transduction, the assays should be useful for identification of inhibitors and activators in high‐throughput screening (HTS) format for drug discovery.