Open AccessLabel‐Free Direct Electrical Detection of a Histidine‐Rich Protein with Sub‐Femtomolar Sensitivity using an Organic Field‐Effect Transistor
Author(s) -
Minamiki Tsukuru,
Sasaki Yui,
Tokito Shizuo,
Minami Tsuyoshi
Publication year - 2017
Publication title -
chemistryopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 29
ISSN - 2191-1363
DOI - 10.1002/open.201700070
Subject(s) - organic field effect transistor , nitrilotriacetic acid , analyte , transistor , electrode , chemistry , field effect transistor , biosensor , materials science , nanotechnology , optoelectronics , chromatography , organic chemistry , electrical engineering , chelation , voltage , engineering
There is a growing interest in achieving sensor systems to enable on‐site testing of biomarkers. Herein, a new strategy for highly sensitive protein detection at sub‐femtomolar levels without any labelling has been demonstrated by using an organic field‐effect transistor (OFET). An artificial histidine‐rich protein receptor (Ni II ‐nitrilotriacetic acid complex, Ni II ‐nta) functionalizes a detection portion (i.e. an extended‐gate electrode) of the fabricated OFET device. The OFET responds electrically and selectively to a target analyte (bovine serum albumin), meaning that the binding processes at the Ni II ‐nta on the extended‐gate electrode for the analyte affect the field‐effect properties of the device. Our results demonstrate that the combination of the OFET with the artificial receptor is an ideal approach for label‐free and immune‐free protein detection.