Open AccessDetermination of lamotrigine in human plasma using liquid chromatography‐tandem mass spectrometry
Author(s) -
Itabashi Shogo,
Bito Rina,
Nishina Maika,
Fukumoto Maki,
Soda Midori,
Doi Mitsunori,
Usui Shigeyuki,
Kitaichi Kiyoyuki
Publication year - 2019
Publication title -
neuropsychopharmacology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.661
H-Index - 13
ISSN - 2574-173X
DOI - 10.1002/npr2.12045
Subject(s) - chromatography , lamotrigine , formic acid , reproducibility , chemistry , therapeutic drug monitoring , protein precipitation , extraction (chemistry) , mass spectrometry , sample preparation , tandem mass spectrometry , liquid chromatography–mass spectrometry , human plasma , calibration curve , pharmacokinetics , pharmacology , detection limit , epilepsy , medicine , psychiatry
Aim Lamotrigine (LTG) is a widely used anti‐epileptic drug that is administered to avoid seizures and to maintain seizure‐free status. However, several factors reportedly cause individual differences of plasma LTG levels, and the therapeutic target range of LTG varies between individuals. Thus, to optimize effective doses of LTG, we developed a rapid and simple method for determining plasma LTG concentrations. Methods Lamotrigine and the internal standard papaverine were extracted from human plasma using solid‐phase extraction. After filtration, 5‐μL aliquots of final samples were injected into the liquid chromatography‐tandem mass spectrometry instrument and LTG and internal standard were separated using a Cadenza CD ‐C18 column (100 × 2 mm, 3 μm) with 0.1% formic acid in water/acetonitrile (2/1, v/v). Results The calibration curve was linear from 0.2 to 5.0 μg/mL, and assessments of recovery, intra‐ and inter‐day precision and accuracy, matrix effects, freeze and thaw stability, and long‐term stability demonstrated good reproducibility. Retention times of LTG and internal standard were 1.6 and 2.0 minutes, respectively, and the total run time was 3.5 minutes for each sample. Conclusion We developed a rapid and simple method for determining plasma LTG concentrations. The present novel system could be used to inform LTG dose adjustments for individual patients.