Premium
Muscle injury in rats induces upregulation of inflammatory cytokines in injured muscle and calcitonin gene–related peptide in dorsal root ganglia innervating the injured muscle
Author(s) -
Sakuma Yoshihiro,
Miyagi Masayuki,
Inoue Gen,
Ishikawa Tetsuhiro,
Kamoda Hiroto,
Yamauchi Kazuyo,
Orita Sumihisa,
Suzuki Miyako,
Oikawa Yasuhiro,
Inage Kazuhide,
Kubota Go,
Sainoh Takeshi,
Sato Jun,
Takahashi Kazuhisa,
Ohtori Seiji
Publication year - 2016
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.25092
Subject(s) - calcitonin gene related peptide , downregulation and upregulation , medicine , dorsal root ganglion , proinflammatory cytokine , h&e stain , gastrocnemius muscle , calcitonin , nerve injury , endocrinology , inflammation , pathology , neuropeptide , anatomy , skeletal muscle , anesthesia , immunohistochemistry , biology , dorsum , receptor , biochemistry , gene
: In this study we evaluated the relationships among the behavioral changes after muscle injury, histological changes, changes in inflammatory cytokines in the injured muscle, and changes in the sensory nervous system innervating the muscle in rats. Methods : We established a model of muscle injury in rats using a dropped weight. Behavior was assessed using the CatWalk system. Subsequently, bilateral gastrocnemius muscles and dorsal root ganglia (DRGs) were resected. Muscles were stained with hematoxylin and eosin, and inflammatory cytokines in injured muscles were assayed. DRGs were immunostained for calcitonin gene–related peptide (CGRP). Results : Changes of behavior and upregulation of inflammatory cytokines in injured muscles subsided within 2 days of injury. Repaired tissue was observed 3 weeks after injury. However, upregulation of CGRP in DRG neurons continued for 2 weeks after injury. Conclusion : These findings may explain in part the pathological mechanism of persistent muscle pain. Muscle Nerve 54 : 776–782, 2016