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Expression of estrogen receptors α and β in the corpus luteum and uterus from non‐pregnant and pregnant llamas
Author(s) -
Powell Susan A.,
Smith Bradford B.,
Timm Karen I.,
Menino Alfred R.
Publication year - 2007
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.20684
Subject(s) - follicular phase , endometrium , luteal phase , biology , corpus luteum , endocrinology , medicine , estrogen receptor , estrogen , uterus , myometrium , estrogen receptor alpha , andrology , estrogen receptor beta , embryo , progesterone receptor , ovary , cancer , breast cancer , microbiology and biotechnology
Abstract Because estrogen may be involved in maternal recognition of pregnancy and embryonic migration in llamas, expression of estrogen receptor subtypes α (ERα) and β (ERβ) was evaluated in corpus luteum (CL), endometrium, and uterus using relative RT‐PCR. Tissues were recovered from sterile‐mated (SM) and pregnant (PG) females during Days 7–11 and 7–13 (Day 0 = day of mating), respectively, and follicular phase and juvenile females. Luteal expression of ERα and β was similar ( P > 0.10) in SM and PG females and within Days 7–11, however, expression of ERα in ovarian tissue from follicular phase females was greater ( P < 0.05) than Days 7 and 9 CL. Uterus expressed less ERα and β compared to endometrium ( P = 0.07 and P < 0.01, respectively). Expression of ERα was greater ( P < 0.05) in Day 7 and follicular phase uteri than Days 9 and 11, Day 13 PG and juvenile uteri. Uterine ERβ expression was greater ( P = 0.09) in PG versus SM females and in mated compared to follicular phase females ( P < 0.05). Endometrial expression of ERα and β did not differ ( P > 0.10) between SM and PG females or by day. The presence of luteal ER during this period may mean a role for estradiol in maternal recognition of pregnancy. Observed increases in uterine ER expression with no changes in endometrium suggest expression increased in myometrium and/or perimetrium. Upregulation of myometrial ERβ in PG females may be involved in supporting uterine migration of the embryo. Mol. Reprod. Dev. 74: 1043–1052, 2007. © 2007 Wiley‐Liss, Inc.