Premium
Development of an extraction method for the determination of avermectins in soil using supercritical CO 2 modified with ethanol and liquid chromatography‐tandem mass spectrometry
Author(s) -
Park JongHyouk,
Choi JeongHeui,
Abd ElAty A. M.,
Park JoonSeong,
Kim Bo Mi,
Na TaeWoong,
Park Ki Hun,
Yang Angel,
Rahman Md. Musfiqur,
Shim JaeHan
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201200600
Subject(s) - chromatography , chemistry , abamectin , avermectin , supercritical fluid extraction , extraction (chemistry) , supercritical fluid , detection limit , mass spectrometry , liquid chromatography–mass spectrometry , moxidectin , calibration curve , ivermectin , pesticide , medicine , zoology , organic chemistry , anatomy , agronomy , biology
The aim of the present study was to develop a multiresidue analytical method for determination of avermectins (abamectin, ivermectin, moxidectin, and doramectin) in soil samples using supercritical fluid extraction and LC‐MS/MS. The optimal extraction conditions for supercritical fluid extraction were 80°C for temperature, 300 kg/cm 2 for pressure, 40 min as an extraction time, and 30% of a modifier ratio. The linearity of the calibration curves was excellent and yielded the correlation coefficients ( r 2 = 0.998–0.999, at a range of 1.5–500 ng/g). Soil samples were fortified with known quantities of the analytes at three different concentration levels (5, 10, and 50 ng/g) and the recoveries were in the range of 82.5–96.2% with relative standard deviation values ranging between 2.1 and 7.9%. The limits of detections and limits of quantitations were 1.5 and 5 ng/g, respectively. The developed method was successfully applied to analyze avermectin residues in soil samples collected from 13 sites in the Honam area, Republic of Korea. In sum, a combination of supercritical fluid extraction and LC‐MS/MS has been proven to be highly efficient as an environmentally friendly technique for the simultaneous determination of avermectins in soil samples.