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Numerical taxonomy of Monascus species based on the electrophoretic analysis of intracellular enzymes
Author(s) -
Nishikawa Jiro,
Iizuka Hiroshi
Publication year - 1993
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620330511
Subject(s) - esterase , electrophoresis , isozyme , lactate dehydrogenase , biology , enzyme , polyacrylamide gel electrophoresis , monascus , chemistry , microbiology and biotechnology , biochemistry , fermentation
Four isozymes in fifteeen species of Monascus were analyzed using polyacrylamide gel electrophoresis. A numerical analysis was carried out on the basis of similarity values obtained from the electrophoretic mobilities of the enzymes. Non‐specific esterase and lactate dehydrogenase showed clearly distinguishable mobilities amont two clusters. From these results obtained, the strains used were divided into two clusters of Group‐I and Group‐II. Both groups were further subdivided into two subclusters. The type strain, M. ruber VAN T IEGHEM MR MO‐10 (IAM 8009) and M. kaoliang MR MO‐F2 were found to belong to the Group‐I. M. pilosus S ATO MR MO‐4 (IAM8003) and M. pubigerus S ATO MR MO‐8 (IAM 8004) were found to belong to the Group‐II. Nearly 100% similarity values were found between M. anka S ATO MR MO‐6 (IAM 8001) and M. rubiginosus S ATO IFO 4484, between M. anka var. rubellus S ATO MR MO‐5 (IAM 8081) and M. purpureus W ENT MR MO‐9 (IAM 8010), and, moreover, among M. vitreus S ATO MR MO‐3 (IAM 8007), M. fuliginosus S ATO IFO 4483 and M. serorubescens S ATO 4487, respectively. Therefore, it was concluded that electrophoretic analysis of enzymes may be useful in presenting a new classification system of Monascus spp.