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DNA fingerprinting of conjugative plasmids incompatible with R6K ( Inc X)
Author(s) -
Prager Rita,
Tietze Erhard,
Tschäpe Helmut
Publication year - 1988
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620280610
Subject(s) - plasmid , dna profiling , chemistry , dna , microbiology and biotechnology , biology , genetics
14 conjugative plasmids originated from different bacterial hosts and geographical sources were identified as members of the incompatibility group Inc X with R6K as the reference plasmid. In spite of their close genetic relatedness (transfer and incompatibility properties), their molecular sizes ranged between 39 and 77 kb and DNA fingerprinting with endonucleases such as Eco RI, Eco RV, Bam HI, Bg /I, Bg /II, Pst I and Sa /I revealed a great variety of fragment patterns.