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Transformation of the industrially important yeasts Candida maltosa and Pichia guilliermondii
Author(s) -
Kunze G.,
Petzoldt C.,
Bode R.,
Samsonova I. A.,
Böttcher F.,
Birnbaum D.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250210
Subject(s) - plasmid , pbr322 , saccharomyces cerevisiae , transformation (genetics) , gene , dna , biology , recombinant dna , genetics , microbiology and biotechnology
Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed at different frequencies by the plasmid p Ye ( ARG4 ) 411 which carried the Sacch. cerevisiae ARG4 gene inserted into pBR322. Our results strongly suggest that the transformants possessed extrachromosomally maintained and autonomously replicating plasmid DNA from which ARG4 gene and β‐lactamase gene were expressed.