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Enzymatische Untersuchungen zur Citrat‐Isocitrat‐Akkumulation bei Hefen mit Glucose als C‐Quelle
Author(s) -
FrankeRinker D.,
Behrens U.,
Nöckel E.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230202
Subject(s) - overproduction , isocitrate dehydrogenase , chemistry , pyruvate carboxylase , aconitase , biochemistry , enzyme , nad+ kinase , citrate synthase , microbiology and biotechnology , biology
The connection between the kinetics of citrate‐isocitrate overproduction by Saccharomycopsis lipolytica in glucose media and the specific activities of the enzymes being related to overproduction has been investigated. The specific activities of citrate synthase, aconitate hydratase, NAD + ‐ linked and NADP + ‐linked isocitrate dehydrogenase decline significantly after exhaustion of the nitrogen source, whereas the activity of the pyruvate carboxylase remains relatively constant and corresponds to changes of the production rate. The results are compared with those obtained by fermentations in n ‐alkane media and discussed in relation to mechanisms of overproduction.