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Production of glucose isomerase by genus Streptomyces
Author(s) -
Suekane M.,
Iizuka H.
Publication year - 1982
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19820220809
Subject(s) - glucose 6 phosphate isomerase , isomerase , streptomyces , fructose , biochemistry , enzyme , streptomycetaceae , strain (injury) , extracellular , xylose isomerase , yield (engineering) , biology , chemistry , actinomycetales , bacteria , genetics , materials science , anatomy , metallurgy
Screening for glucose isomerases was done using 46 species and 63 strains of Streptomyces type cultures. Glucose isomerase was found in a wide range of these species. The intracellular glucose isomerase of S. olivochromogenes showed the highest fructose conversion ratio. The apparent extracellular glucose isomerase activities were observed for several strains of Streptomyces, Streptomyces venezuelae had the highest apparent extracellular glucose isomerase activity. The glucose isomerase yield was improved from 1.95 units/ml to 7.5 units/ml by induced mutation and culturing condition studies. This increase isomerase yield has made it possible to produce glucose isomerase industrially.