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Untersuchungen an T3‐Phagen. VII. Cross streak‐Komplementierung von temperatursensitiven
Author(s) -
Krüger D. H.,
Scholz D.,
Rosenthal H. A.
Publication year - 1975
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19750150203
Subject(s) - complementation , mutagenesis , streak , mutant , microbiology and biotechnology , biology , genetics , gene , chemistry , mineralogy
Temperature‐sensitive ( ts ) mutants of phage T3 were isolated following mutagenesis with nitrosoguanidine. We have found 9 complementation groups by means of the cross streak method applied in an incubator which maintains a constant non‐permissive temperature. The same method permits the rapid recognition of ts mutations in previously defined genes immediately after mutagenesis.