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An efficient method for following the enzymic reactions involved in camphor biosynthesis in cinnamomum camphora by use of GC‐MS and regiospecifically deuteriated substrate
Author(s) -
Ohta Shinji,
Yamamitsu Tohru,
Suga Takayuki
Publication year - 1992
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580310509
Subject(s) - camphor , cinnamomum camphora , chemistry , borneol , biosynthesis , substrate (aquarium) , stereochemistry , chromatography , organic chemistry , enzyme , medicine , oceanography , alternative medicine , traditional chinese medicine , pathology , geology
Abstract An efficient method has been developed to follow the enzymic reactions involved in the biosynthesis of camphor in Cinnamomum camphora (camphor tree) by use of [5,5‐ 2 H 2 ]geranyl diphosphate as a substrate and GC‐MS with selected ion monitoring. Borneol and camphor biosynthesized in the enzymic reactions gave a base peak due to the deuterium‐containing ion in its EI mass spectrum. It is possible to detect 1.5 ng of the biosynthesized borneol and camphor per injection into the GC‐MS. This method enabled us to differentiate easily the biosynthesized camphor from the endogenous camphor and it is a facile and sensitive technique to determine the amount of the biosynthesized camphor.