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Ein Verfahren zur Herstellung von Mehrfach mit Tritium Markiertem Thymin BZW. Thymidin
Author(s) -
Pleiß U.,
Römer J.
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580201207
Subject(s) - chemistry , thymine , tritium , thymidine , radiochemistry , nuclear chemistry , biochemistry , dna , physics , nuclear physics
After a survey about the preparation of tritium labelled thymine and thymidine a new synthesis for the preparation of manifold labelled thymine respectively thymidine is given. 5‐Formyluracil was reduced to 5‐hydroxymethyluracil (5‐HMU) in a phosphate buffer at pH 7,0 with tritium gas using Pd0/BaSO 4 as catalyst. The tritium labelled 5‐HMU was hydrogenated to [ 4 methyl‐ 3 H] thymine with tritium gas using a mixture of Palladium black and Palladiumchloride in dry dioxane. The specific activity was 2550 GBq/mmol. [methyl‐ 3 H] Thymidine was obtained in a usual enzymatic synthesis.