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Degradation of 14 C‐L‐histidine during chromatography and electrophoresis
Author(s) -
Hrnčíř Š,
Kopoldové J.,
Vereš K.,
Dědkové V.,
Hanuš V.,
Sedmera P.
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580150108
Subject(s) - chemistry , histidine , degradation (telecommunications) , chromatography , formaldehyde , thin layer chromatography , condensation , electrophoresis , organic chemistry , telecommunications , physics , computer science , thermodynamics , enzyme
The loss of histidine and the formation of additional by‐products during the purification and storage of [ 14 C(U)]‐L‐histidine preparations on chromatographic carriers was followed. The product formed mainly on chromatographic paper was identified as the condensation product of histidine with formaldehyde; on thin layer of silica gel mainly degradation products of histidine were obtained. Possibilities to decrease the formation of these undesirable by‐products were discussed.