Open AccessPersister cells as a possible cause of antibiotic therapy failure in Helicobacter pylori
Author(s) -
Bahmaninejad Parvin,
Ghafourian Sobhan,
Mahmoudi Mina,
Maleki Abbas,
Sadeghifard Nourkhoda,
Badakhsh Behzad
Publication year - 2021
Publication title -
jgh open
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 8
ISSN - 2397-9070
DOI - 10.1002/jgh3.12527
Subject(s) - multidrug tolerance , clarithromycin , antibiotics , helicobacter pylori , medicine , microbiology and biotechnology , minimum inhibitory concentration , biopsy , gastroenterology , antibiotic therapy , bacteria , biology , biofilm , genetics
Background and Aim Due to the failure of antibiotic treatment and recurrence of infection in patients with Helicobacter pylori , this study was designed to find the possible cause of treatment failure and recurrence of the H. pylori infections in Ilam, Iran. Methods One hundred patients with specific symptoms of H. pylori infection were selected, and after taking a biopsy specimen, identification of H. pylori , antibiotic susceptibility assay, and persister cell assay were performed. In addition, after treatment, patients with persister cells were followed for possible recurrence of infection. Furthermore, an antibiotic susceptibility assay was performed. Results Our results demonstrated that, among 100 patients, 50% (n = 50) showed positive results for the existence of H. pylori . Among the susceptible isolates, 18% (n = 9) were persister cells that were sensitive to clarithromycin as confirmed by a 5 folds higher than the Minimal Inhibitory Concentration (MIC) of clarithromycin. The data were confirmed by following up the suspected patients. Conclusion Our results demonstrated that persister cells in H. pylori infections may be responsible to recurrent infection and antibiotic treatment failure. However, more research is needed to obtain more information in this area.