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Intracellular uranium distribution: Comparison of cryogenic fixation versus chemical fixation methods for SIMS analysis
Author(s) -
Suhard D.,
Tessier C.,
Manens L.,
Rebière F.,
Tack K.,
Agarande M.,
Guéguen Y.
Publication year - 2018
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.23047
Subject(s) - uranium , secondary ion mass spectrometry , cytoplasm , chemistry , radiochemistry , fixation (population genetics) , transmission electron microscopy , intracellular , nucleus , biophysics , analytical chemistry (journal) , mass spectrometry , materials science , nanotechnology , biochemistry , biology , environmental chemistry , chromatography , microbiology and biotechnology , metallurgy , gene
Abstract Localization of uranium within cells is mandatory for the comprehension of its cellular mechanism of toxicity. Secondary Ion Mass Spectrometry (SIMS) has recently shown its interest to detect and localize uranium at very low levels within the cells. This technique requires a specific sample preparation similar to the one used for Transmission Electronic Microscopy, achieved by implementing different chemical treatments to preserve as much as possible the living configuration uranium distribution into the observed sample. This study aims to compare the bioaccumulation sites of uranium within liver or kidney cells after chemical fixation and cryomethods preparations of the samples: SIMS analysis of theses samples show the localization of uranium soluble forms in the cell cytoplasm and nucleus with a more homogenous distribution when using cryopreparation probably due to the diffusible portion of uranium inside the cytoplasm.