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BACKGROUND  Phytohormones are chemical messengers that have a positive effect on biodiesel production of microalgae at low concentrations. However, the effect of phytohormone 6‐benzylaminopurine on lipid and docosahexaenoic acid ( DHA ) production in marine  DHA ‐producer  Aurantiochytrium  has never been reported. In this study, a  GC‐MS ‐based metabolomics method combined with a multivariate analysis is applied to reveal the metabolic mechanism of 6‐benzylaminopurine enhancing production of lipid and  DHA  in  Aurantiochytrium  sp. YLH70 .    RESULTS  In total, 71 metabolites were identified by  GC‐MS . The  PCA  model revealed that 76.9% of metabolite variation was related to 6‐benzylaminopurine treatment, and overall metabolomics profiles between the 6‐benzylaminopurine and control groups were clearly discriminated. Forty‐six metabolites identified by the  PLS‐DA  model were responsible for responding to 6‐benzylaminopurine. Metabolic analysis showed that 6‐benzylaminopurine could accelerate the rate of utilization of glucose in  Aurantiochytrium  sp.  YLH70 , and the metabolic flux from glycolysis,  TCA  cycle and mevalonate pathway to fatty acids biosynthesis was promoted. Moreover, the anti‐stress mechanism in  Aurantiochytrium  sp. YLH70  might be induced by 6‐benzylaminopurine.    CONCLUSION  Metabolomics is a suitable tool to discover the metabolic mechanism for improving lipid and  DHA  accumulation in a microorganism. 6‐benzylaminopurine has the potential to stimulate lipid and  DHA  production of  Aurantiochytrium  sp. YLH70  for industrial purposes. © 2015 The Authors.  Journal of Chemical Technology & Biotechnology  published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Metabolomics analysis reveals 6‐benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytrium sp.